Functional genomics highlights differential induction of antiviral pathways in the lungs of SARS-CoV-infected macaques.
Identifieur interne : 003701 ( Main/Exploration ); précédent : 003700; suivant : 003702Functional genomics highlights differential induction of antiviral pathways in the lungs of SARS-CoV-infected macaques.
Auteurs : Anna De Lang [Pays-Bas] ; Tracey Baas ; Thomas Teal ; Lonneke M. Leijten ; Brandon Rain ; Albert D. Osterhaus ; Bart L. Haagmans ; Michael G. KatzeSource :
- PLoS pathogens [ 1553-7374 ] ; 2007.
Descripteurs français
- KwdFr :
- Analyse de profil d'expression de gènes, Animaux, Cytokines (génétique), Cytokines (métabolisme), Facteur de transcription STAT-1 (métabolisme), Génomique, Immunohistochimie, Interféron de type I (biosynthèse), Interféron de type I (génétique), Macaca fascicularis, Marqueurs biologiques (métabolisme), Modèles animaux de maladie humaine, Poumon (anatomopathologie), Poumon (immunologie), Poumon (virologie), Régulation de l'expression des gènes viraux, Réplication virale, Syndrome respiratoire aigu sévère (anatomopathologie), Syndrome respiratoire aigu sévère (métabolisme), Syndrome respiratoire aigu sévère (virologie), Séquençage par oligonucléotides en batterie, Virus du SRAS (génétique), Virus du SRAS (pathogénicité).
- MESH :
- anatomopathologie : Poumon, Syndrome respiratoire aigu sévère.
- biosynthèse : Interféron de type I.
- génétique : Cytokines, Interféron de type I, Virus du SRAS.
- immunologie : Poumon.
- métabolisme : Cytokines, Facteur de transcription STAT-1, Marqueurs biologiques, Syndrome respiratoire aigu sévère.
- pathogénicité : Virus du SRAS.
- virologie : Poumon, Syndrome respiratoire aigu sévère.
- Analyse de profil d'expression de gènes, Animaux, Génomique, Immunohistochimie, Macaca fascicularis, Modèles animaux de maladie humaine, Régulation de l'expression des gènes viraux, Réplication virale, Séquençage par oligonucléotides en batterie.
English descriptors
- KwdEn :
- Animals, Biomarkers (metabolism), Cytokines (genetics), Cytokines (metabolism), Disease Models, Animal, Gene Expression Profiling, Gene Expression Regulation, Viral, Genomics, Immunohistochemistry, Interferon Type I (biosynthesis), Interferon Type I (genetics), Lung (immunology), Lung (pathology), Lung (virology), Macaca fascicularis, Oligonucleotide Array Sequence Analysis, SARS Virus (genetics), SARS Virus (pathogenicity), STAT1 Transcription Factor (metabolism), Severe Acute Respiratory Syndrome (metabolism), Severe Acute Respiratory Syndrome (pathology), Severe Acute Respiratory Syndrome (virology), Virus Replication.
- MESH :
- chemical , biosynthesis : Interferon Type I.
- chemical , genetics : Cytokines, Interferon Type I.
- chemical , metabolism : Biomarkers, Cytokines, STAT1 Transcription Factor.
- genetics : SARS Virus.
- immunology : Lung.
- metabolism : Severe Acute Respiratory Syndrome.
- pathogenicity : SARS Virus.
- pathology : Lung, Severe Acute Respiratory Syndrome.
- virology : Lung, Severe Acute Respiratory Syndrome.
- Animals, Disease Models, Animal, Gene Expression Profiling, Gene Expression Regulation, Viral, Genomics, Immunohistochemistry, Macaca fascicularis, Oligonucleotide Array Sequence Analysis, Virus Replication.
Abstract
The pathogenesis of severe acute respiratory syndrome coronavirus (SARS-CoV) is likely mediated by disproportional immune responses and the ability of the virus to circumvent innate immunity. Using functional genomics, we analyzed early host responses to SARS-CoV infection in the lungs of adolescent cynomolgus macaques (Macaca fascicularis) that show lung pathology similar to that observed in human adults with SARS. Analysis of gene signatures revealed induction of a strong innate immune response characterized by the stimulation of various cytokine and chemokine genes, including interleukin (IL)-6, IL-8, and IP-10, which corresponds to the host response seen in acute respiratory distress syndrome. As opposed to many in vitro experiments, SARS-CoV induced a wide range of type I interferons (IFNs) and nuclear translocation of phosphorylated signal transducer and activator of transcription 1 in the lungs of macaques. Using immunohistochemistry, we revealed that these antiviral signaling pathways were differentially regulated in distinctive subsets of cells. Our studies emphasize that the induction of early IFN signaling may be critical to confer protection against SARS-CoV infection and highlight the strength of combining functional genomics with immunohistochemistry to further unravel the pathogenesis of SARS.
DOI: 10.1371/journal.ppat.0030112
PubMed: 17696609
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 001D46
- to stream PubMed, to step Curation: 001D46
- to stream PubMed, to step Checkpoint: 001E05
- to stream Ncbi, to step Merge: 001A40
- to stream Ncbi, to step Curation: 001A40
- to stream Ncbi, to step Checkpoint: 001A40
- to stream Main, to step Merge: 003842
- to stream Main, to step Curation: 003701
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Functional genomics highlights differential induction of antiviral pathways in the lungs of SARS-CoV-infected macaques.</title><author><name sortKey="De Lang, Anna" sort="De Lang, Anna" uniqKey="De Lang A" first="Anna" last="De Lang">Anna De Lang</name><affiliation wicri:level="3"><nlm:affiliation>Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Virology, Erasmus Medical Center, Rotterdam</wicri:regionArea><placeName><settlement type="city">Rotterdam</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Baas, Tracey" sort="Baas, Tracey" uniqKey="Baas T" first="Tracey" last="Baas">Tracey Baas</name></author><author><name sortKey="Teal, Thomas" sort="Teal, Thomas" uniqKey="Teal T" first="Thomas" last="Teal">Thomas Teal</name></author><author><name sortKey="Leijten, Lonneke M" sort="Leijten, Lonneke M" uniqKey="Leijten L" first="Lonneke M" last="Leijten">Lonneke M. Leijten</name></author><author><name sortKey="Rain, Brandon" sort="Rain, Brandon" uniqKey="Rain B" first="Brandon" last="Rain">Brandon Rain</name></author><author><name sortKey="Osterhaus, Albert D" sort="Osterhaus, Albert D" uniqKey="Osterhaus A" first="Albert D" last="Osterhaus">Albert D. Osterhaus</name></author><author><name sortKey="Haagmans, Bart L" sort="Haagmans, Bart L" uniqKey="Haagmans B" first="Bart L" last="Haagmans">Bart L. Haagmans</name></author><author><name sortKey="Katze, Michael G" sort="Katze, Michael G" uniqKey="Katze M" first="Michael G" last="Katze">Michael G. Katze</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2007">2007</date><idno type="RBID">pubmed:17696609</idno><idno type="pmid">17696609</idno><idno type="doi">10.1371/journal.ppat.0030112</idno><idno type="wicri:Area/PubMed/Corpus">001D46</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001D46</idno><idno type="wicri:Area/PubMed/Curation">001D46</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001D46</idno><idno type="wicri:Area/PubMed/Checkpoint">001E05</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001E05</idno><idno type="wicri:Area/Ncbi/Merge">001A40</idno><idno type="wicri:Area/Ncbi/Curation">001A40</idno><idno type="wicri:Area/Ncbi/Checkpoint">001A40</idno><idno type="wicri:Area/Main/Merge">003842</idno><idno type="wicri:Area/Main/Curation">003701</idno><idno type="wicri:Area/Main/Exploration">003701</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Functional genomics highlights differential induction of antiviral pathways in the lungs of SARS-CoV-infected macaques.</title><author><name sortKey="De Lang, Anna" sort="De Lang, Anna" uniqKey="De Lang A" first="Anna" last="De Lang">Anna De Lang</name><affiliation wicri:level="3"><nlm:affiliation>Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Virology, Erasmus Medical Center, Rotterdam</wicri:regionArea><placeName><settlement type="city">Rotterdam</settlement><region nuts="2" type="province">Hollande-Méridionale</region></placeName></affiliation></author><author><name sortKey="Baas, Tracey" sort="Baas, Tracey" uniqKey="Baas T" first="Tracey" last="Baas">Tracey Baas</name></author><author><name sortKey="Teal, Thomas" sort="Teal, Thomas" uniqKey="Teal T" first="Thomas" last="Teal">Thomas Teal</name></author><author><name sortKey="Leijten, Lonneke M" sort="Leijten, Lonneke M" uniqKey="Leijten L" first="Lonneke M" last="Leijten">Lonneke M. Leijten</name></author><author><name sortKey="Rain, Brandon" sort="Rain, Brandon" uniqKey="Rain B" first="Brandon" last="Rain">Brandon Rain</name></author><author><name sortKey="Osterhaus, Albert D" sort="Osterhaus, Albert D" uniqKey="Osterhaus A" first="Albert D" last="Osterhaus">Albert D. Osterhaus</name></author><author><name sortKey="Haagmans, Bart L" sort="Haagmans, Bart L" uniqKey="Haagmans B" first="Bart L" last="Haagmans">Bart L. Haagmans</name></author><author><name sortKey="Katze, Michael G" sort="Katze, Michael G" uniqKey="Katze M" first="Michael G" last="Katze">Michael G. Katze</name></author></analytic><series><title level="j">PLoS pathogens</title><idno type="eISSN">1553-7374</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Biomarkers (metabolism)</term><term>Cytokines (genetics)</term><term>Cytokines (metabolism)</term><term>Disease Models, Animal</term><term>Gene Expression Profiling</term><term>Gene Expression Regulation, Viral</term><term>Genomics</term><term>Immunohistochemistry</term><term>Interferon Type I (biosynthesis)</term><term>Interferon Type I (genetics)</term><term>Lung (immunology)</term><term>Lung (pathology)</term><term>Lung (virology)</term><term>Macaca fascicularis</term><term>Oligonucleotide Array Sequence Analysis</term><term>SARS Virus (genetics)</term><term>SARS Virus (pathogenicity)</term><term>STAT1 Transcription Factor (metabolism)</term><term>Severe Acute Respiratory Syndrome (metabolism)</term><term>Severe Acute Respiratory Syndrome (pathology)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Virus Replication</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term><term>Animaux</term><term>Cytokines (génétique)</term><term>Cytokines (métabolisme)</term><term>Facteur de transcription STAT-1 (métabolisme)</term><term>Génomique</term><term>Immunohistochimie</term><term>Interféron de type I (biosynthèse)</term><term>Interféron de type I (génétique)</term><term>Macaca fascicularis</term><term>Marqueurs biologiques (métabolisme)</term><term>Modèles animaux de maladie humaine</term><term>Poumon (anatomopathologie)</term><term>Poumon (immunologie)</term><term>Poumon (virologie)</term><term>Régulation de l'expression des gènes viraux</term><term>Réplication virale</term><term>Syndrome respiratoire aigu sévère (anatomopathologie)</term><term>Syndrome respiratoire aigu sévère (métabolisme)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Séquençage par oligonucléotides en batterie</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (pathogénicité)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Interferon Type I</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Cytokines</term><term>Interferon Type I</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Biomarkers</term><term>Cytokines</term><term>STAT1 Transcription Factor</term></keywords><keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Poumon</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Interféron de type I</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Cytokines</term><term>Interféron de type I</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Poumon</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Lung</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cytokines</term><term>Facteur de transcription STAT-1</term><term>Marqueurs biologiques</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Lung</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Poumon</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Lung</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Disease Models, Animal</term><term>Gene Expression Profiling</term><term>Gene Expression Regulation, Viral</term><term>Genomics</term><term>Immunohistochemistry</term><term>Macaca fascicularis</term><term>Oligonucleotide Array Sequence Analysis</term><term>Virus Replication</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term><term>Animaux</term><term>Génomique</term><term>Immunohistochimie</term><term>Macaca fascicularis</term><term>Modèles animaux de maladie humaine</term><term>Régulation de l'expression des gènes viraux</term><term>Réplication virale</term><term>Séquençage par oligonucléotides en batterie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The pathogenesis of severe acute respiratory syndrome coronavirus (SARS-CoV) is likely mediated by disproportional immune responses and the ability of the virus to circumvent innate immunity. Using functional genomics, we analyzed early host responses to SARS-CoV infection in the lungs of adolescent cynomolgus macaques (Macaca fascicularis) that show lung pathology similar to that observed in human adults with SARS. Analysis of gene signatures revealed induction of a strong innate immune response characterized by the stimulation of various cytokine and chemokine genes, including interleukin (IL)-6, IL-8, and IP-10, which corresponds to the host response seen in acute respiratory distress syndrome. As opposed to many in vitro experiments, SARS-CoV induced a wide range of type I interferons (IFNs) and nuclear translocation of phosphorylated signal transducer and activator of transcription 1 in the lungs of macaques. Using immunohistochemistry, we revealed that these antiviral signaling pathways were differentially regulated in distinctive subsets of cells. Our studies emphasize that the induction of early IFN signaling may be critical to confer protection against SARS-CoV infection and highlight the strength of combining functional genomics with immunohistochemistry to further unravel the pathogenesis of SARS.</div></front></TEI><affiliations><list><country><li>Pays-Bas</li></country><region><li>Hollande-Méridionale</li></region><settlement><li>Rotterdam</li></settlement></list><tree><noCountry><name sortKey="Baas, Tracey" sort="Baas, Tracey" uniqKey="Baas T" first="Tracey" last="Baas">Tracey Baas</name><name sortKey="Haagmans, Bart L" sort="Haagmans, Bart L" uniqKey="Haagmans B" first="Bart L" last="Haagmans">Bart L. Haagmans</name><name sortKey="Katze, Michael G" sort="Katze, Michael G" uniqKey="Katze M" first="Michael G" last="Katze">Michael G. Katze</name><name sortKey="Leijten, Lonneke M" sort="Leijten, Lonneke M" uniqKey="Leijten L" first="Lonneke M" last="Leijten">Lonneke M. Leijten</name><name sortKey="Osterhaus, Albert D" sort="Osterhaus, Albert D" uniqKey="Osterhaus A" first="Albert D" last="Osterhaus">Albert D. Osterhaus</name><name sortKey="Rain, Brandon" sort="Rain, Brandon" uniqKey="Rain B" first="Brandon" last="Rain">Brandon Rain</name><name sortKey="Teal, Thomas" sort="Teal, Thomas" uniqKey="Teal T" first="Thomas" last="Teal">Thomas Teal</name></noCountry><country name="Pays-Bas"><region name="Hollande-Méridionale"><name sortKey="De Lang, Anna" sort="De Lang, Anna" uniqKey="De Lang A" first="Anna" last="De Lang">Anna De Lang</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003701 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003701 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:17696609
|texte= Functional genomics highlights differential induction of antiviral pathways in the lungs of SARS-CoV-infected macaques.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:17696609" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |